It will come up in dialogue. It came up for me with Ashley when I talked with her. Yes she does but the two years apart have changed her dramatically. Her personality has taken a darker tone and she is not very emotional when you meet her.
She will acknowledge it (will not spoil how exactly), but the circumstances (the fact that all her words are probably recorded while she is on Illium, her devotion to her "hunt", and her sense of guilt for giving Shepard to the ruthless Cerberus) will prevent her from giving into her feelings for more than just a few brief moments.